| NT-ProANP

Amino-terminal pro-cardiac natriuretic peptide (NT-ProANP) is the N-terminal polypeptide of the ANP precursor. In the rough vesicles of atrial cells, a 149 amino acid residue of pro-ANP is first produced, and its signal peptide (23 amino acids) is cut off in the Golgi, leaving a 126 amino acid pro-ANP that is stored in secretory granules. Prior to secretion, the 98 amino acid N-terminal peptide (NT-pro ANP) and the 28 amino acid C-terminal peptide (ANP) were separated. ANP is secreted from the atria as a result of atrial dilation stimulated by increased blood volume and/or increased intra-atrial pressure.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	2500	1250	625	312.5	156.25	78.12	39.06	0
OD	2.34	1.66	1.07	0.68	0.43	0.32	0.23	0.1
Corrected OD	2.24	1.56	0.97	0.58	0.33	0.22	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human NT-ProANP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	93-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human NT-ProANP and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108